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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
Quantigene Bdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
Quantigene 2.0 Bdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantigene 2.0 bdna assay kit/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
quantigene 2.0 bdna assay kit - by Bioz Stars, 2026-04
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Thermo Fisher quantigene 2 0 bdna assay kit
Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
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Panomics Inc quantigene i explore kit for bdna
Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by <t>bDNA</t> assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.
Quantigene I Explore Kit For Bdna, supplied by Panomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by bDNA assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Self-Delivering RNAi Compounds for Reduction of Hyperpigmentation

doi: 10.2147/CCID.S498987

Figure Lengend Snippet: Hit compounds identified in the original screen underwent further chemical optimization to enhance potency and stability. The resulting optimized compounds were screened with the original compounds for comparison. Screening was performed in SK-MEL-5 cells using 5-point dose response curves. TYR mRNA levels were assessed by bDNA assay ( A ). Confirmation of the two best hits from the SK-MEL-5 testing was performed in primary human melanocytes (HEMn) ( B ). Opt 3 was selected as the lead compound and renamed RXI-231.

Article Snippet: For confirmation of hit compounds, TYR mRNA expression levels were measured using the QuantiGene bDNA assay kit (ThermoFisher).

Techniques: Comparison